Menene Gwajin Endotoxins?

Menene Gwajin Endotoxins?

Endotoxins sune kwayoyin hydrophobic wadanda ke cikin hadaddun lipopolysaccharide wanda ke samar da mafi yawan kwayar cutar Gram-korau.Ana sakin su ne lokacin da ƙwayoyin cuta suka mutu kuma membranes na waje ya tarwatse.Ana ɗaukar Endotoxins a matsayin manyan masu ba da gudummawa ga amsawar pyrogenic.Kuma samfuran mahaifa da aka gurbata da pyrogens na iya haifar da haɓakar zazzabi, ƙaddamar da amsa mai kumburi, girgiza, gazawar gabbai da mutuwa a cikin mutane.

Gwajin Endotoxins shine gwajin gano ko ƙididdige endotoxins daga ƙwayoyin cuta na Gram-korau.

Ana amfani da zomaye don ganowa da ƙididdige adadin endotoxins a cikin samfuran magunguna da farko.A cewar USP, RPT ta ƙunshi saka idanu don haɓakar zafin jiki ko zazzabi bayan allurar ta hanyar jijiya na magunguna cikin zomaye.Kuma 21 CFR 610.13(b) yana buƙatar gwajin pyrogen zomo don ƙayyadaddun samfuran halitta.

A cikin 1960s, Fredrick Bang da Jack Levin sun gano cewa amebocytes na kaguwar doki za su toshe a gaban endotoxins.TheLimulus Amebocyte Lysate(ko Tachypleus Amebocyte Lysate) an haɓaka shi daidai don maye gurbin yawancin RPT.A kan USP, gwajin LAL ana kiransa gwajin endotoxin na kwayan cuta (BET).Kuma ana iya yin BET ta amfani da dabaru guda 3: 1) dabarar gel-clot;2) dabarar turbidimetric;3) fasahar chromogenic.Abubuwan buƙatu don gwajin LAL sun ƙunshi mafi kyawun pH, ƙarfin ionic, zafin jiki, da lokacin shiryawa.

Idan aka kwatanta da RPT, BET yana da sauri da inganci.Koyaya, BET ba zai iya maye gurbin RPT gaba ɗaya ba.Saboda gwaje-gwajen LAL na iya shiga tsakani da dalilai kuma ba zai iya gano pyrogens marasa endotoxin ba.