Recombinant Factor C (rFC) assaywata hanya ce da aka fi amfani da ita don gano endotoxins na kwayan cuta, wanda kuma aka sani da lipopolysaccharides (LPS), Endotoxins wani bangare ne na jikin kwayar cutar Gram-korau wanda zai iya haifar da amsa mai zafi a cikin dabbobi, ciki har da mutane.Ƙididdigar rFC ta dogara ne akan amfani da wani nau'i na nau'i na Factor C, wani enzyme wanda aka samo shi a cikin jinin kaguwar doki kuma yana shiga cikin hanyar clotting.A cikin kimar rFC, ana amfani da recombinant Factor C don gano kasancewar endotoxins ta hanyar aunawa Ta hanyar auna abun ciki na tarkace a gaban endotoxin.Idan aka kwatanta da hanyoyin gargajiya na gano endotoxin, irin su Limulus Amebocyte Lysate (LAL) assay wanda ke amfani da jinin kaguwar doki, rFC assay ana ɗaukarsa ya zama mafi daidaitacce kuma ana iya sake haifuwa, saboda baya dogara ga amfani da reagents na dabba.Binciken rFC kuma ya fi dacewa da muhalli da dorewa, saboda yana rage buƙatar tattarawa da amfani da kaguwar doki a cikin gano endotoxin.
Ƙididdigar rFC ta sami amincewa da hukumomin gudanarwa, irin su Amurka Pharmacopeia (USP), European Pharmacopoeia (EP) da Pharmacopoeia na China (CP) don amfani da gwajin kula da ingancin magunguna da na'urorin likita.
Amfanin recombinant factor c assay
Ƙididdigar Factor Factor C (rFC) tana ba da fa'idodi da yawa akan hanyoyin gargajiya don gano endotoxins, kamar gwajin Limulus Amebocyte Lysate (LAL).Wasu fa'idodin rFC assay sun haɗa da:
1. Daidaitawa: Ƙididdigar rFC fasaha ce ta DNA ta sake haɗawa da ke amfani da furotin guda ɗaya, da aka ƙayyade a matsayin mai ganowa.Wannan yana sa ƙididdigar ta fi daidaitawa kuma ba ta da sauƙi ga sauye-sauye idan aka kwatanta da gwajin LAL, wanda ya dogara da amfani da hadadden cakuda sunadarai da aka ciro daga jinin kaguwar doki.
2. Reproducibility: Ƙididdigar rFC tana da matakan haɓakawa masu yawa, kamar yadda yake amfani da furotin guda ɗaya, da aka ƙayyade a matsayin mai ganowa.Wannan yana ba da damar samun daidaiton sakamako, har ma a cikin batches daban-daban da yawa na reagents.
3. Rage yawan amfani da dabba: Ƙididdigar rFC ita ce mafi dacewa da muhalli kuma hanya mai dorewa don gano endotoxins, kamar yadda ba ya buƙatar amfani da dabbobi masu rai ko sadaukarwa, irin su kaguwar doki.
4. Mai tsada: Ƙididdigar rFC gabaɗaya ta fi tasiri fiye da ƙimar LAL, saboda rage buƙatar dabbobi masu rai da kuma mafi daidaitaccen yanayin kima.
5. Kwanciyar hankali: Gwajin rFC yana da ƙarfi kuma ana iya amfani dashi a cikin aikace-aikace masu yawa, ciki har da gwajin kula da ingancin magunguna, na'urorin likita, da sauran samfurori waɗanda zasu iya ƙunsar endotoxins.
6. Amincewa da ka'ida: An amince da gwajin rFC ta hanyar hukumomin gudanarwa irin su Amurka Pharmacopeia (USP), Pharmacopoeia na Turai (EP) da Pharmacopoeia na kasar Sin (CP) don amfani da gwajin kula da ingancin magunguna da na'urorin likitanci.Wannan yana ba da babban matakin amincewa da aminci da daidaito na kima.
Don saduwa da buƙatu iri-iri, Bioendo kuma yana samarwa kuma yana ba da hanyar gargajiya na kayan aikin gwaji na gel clot endotoxin, kayan saurin gel clot assay kit, kit ɗin gwajin gwajin endotoxin mai ƙima gami da "Kinetic turbidimetric endotoxin test assay kitkumaKinetic chromogenic endotoxin gwajin kit” .
Lokacin aikawa: Fabrairu-19-2023
