Gilashin gilashi tare da sarrafa depyrogenation wajibi ne a cikin gwajin gwajin endotoxin don tabbatar da daidaito da amincin sakamakon gwajin.Endotoxins su ne abubuwan da ke da ƙarfi da ƙarfi daga bangon tantanin waje na wasu ƙwayoyin cuta na gram-korau, kuma suna iya haifar da rashin lafiya mai tsanani har ma da mutuwa a cikin mutane idan suna cikin samfuran likita ko na'urori.
Don gano endotoxins, gwajin yana amfani da reagents wanda ya ƙunshi Limulus Amebocyte Lysate (LAL) ko kuma ana kiransa Lyophilized amebocyte lysate, wani tsantsa daga sel jini na kaguwar doki wanda ke da tsarin clotting wanda endotoxins ke kunnawa.Koyaya, bututun gilashin da ba a cire su ba na iya tsoma baki tare da gwajin gwajin LAL ta hanyar kunna tsarin clotting ɗin sa da kuma samar da sakamako mai kyau na ƙarya.Don haka, bututun gilashin da aka yi amfani da su a cikin gwajin gwajin endotoxin dole ne a lalata su don cire duk wani endotoxins wanda zai iya kasancewa kuma don hana kunna reagent na LAL.Wannan yana tabbatar da cewa sakamakon gwajin endotoxin daidai ne kuma abin dogaro kuma ba a fallasa marasa lafiya zuwa matakan cutarwa na endotoxins.da kuma tabbatar da lafiyar magungunan mahaifa a cikin magunguna, furotin, al'adun tantanin halitta, DNA da sauransu.
Lalacewar bututun gilashi marasa endotoxin da ake amfani da su a cikin aikin tantancewar endotoxin:
Bututun gilashi marasa kyauta na Endotoxinsu ne muhimmin sashi na kowane gwajin gwajin endotoxin.An tsara waɗannan bututun gilashin don rage haɗarin gurɓatawar endotoxin yayin aikin gwaji, tabbatar da ingantaccen sakamako mai inganci.
Ɗaya daga cikin mahimman fasalulluka na bututun gilashi marasa endotoxin shine tsarin sinadaran su.An yi waɗannan bututun daga gilashin borosilicate mai inganci, wanda aka sani don juriya na musamman ga lalata sinadarai.Wannan ya sa su dace don amfani da su a cikin gwajin endotoxin, saboda za su iya jure wa ɗaukar hoto zuwa nau'i-nau'i masu yawa na gwaji ba tare da lalata ko gurɓata samfurin ba.
Wani muhimmin fasali na bututun gilashi marasa endotoxin shine tsabtarsu.Ana tsabtace waɗannan bututun a hankali kuma ana haifuwa kafin amfani da su don cire duk wata hanyar da za ta iya haifar da gurɓata.Hakanan ana gwada su da ƙarfi don gurɓatawar endotoxin, tare da tabbatar da cewa sun sami 'yanci daga kowane adadin wannan abu mai cutarwa.
Bugu da ƙari, an tsara bututun gilashi marasa endotoxin don sauƙin amfani.Yawanci ana samun su a cikin kewayon masu girma dabam da daidaitawa don ɗaukar nau'ikan samfuran samfuri daban-daban da hanyoyin gwaji duka gwajin gwajin endotoxin na inganci da ƙimar gwajin gwaji na endotoxin.Har ila yau, sun dace da nau'o'in shirye-shiryen samfurin da kayan gwaji, suna mai da su zabi mai mahimmanci da dacewa don dakunan gwaje-gwaje na endotoxin.
Gabaɗaya, bututun gilashi marasa endotoxin suna taka muhimmiyar rawa a cikin daidaito da amincin gwajin endotoxin.Babban ingancin gininsu, tsabta, da sauƙin amfani ya sa su zama muhimmin sashi na kowane ingantaccen gwajin gwajin endotoxin.
Bioendo Endotoxin-free gilashin bututu tare da girma10*75mm, 12*75mm, 13*100mm da 16*100mmdon hanyoyin dilutions da hanyoyin amsawa.
Bututun gilashin da ba su da Endotoxin sun dace da daidaitattun matakin endotoxins ƙasa da 0.005EU/ml.
Ya kamata a yi amfani da bututun gilashi marasa kyauta na endotoxin a cikin gwajin gwajin endotoxin na gel clot don hana sakamako mara kyau.
Endotoxins sune sassan bangon sel na kwayan cuta waɗanda zasu iya gurɓata kayan aikin dakin gwaje-gwaje, gami da bututun gilashi.
Ana amfani da gwajin gwajin endotoxin na gel don gano kasancewar endotoxins a cikin samfurin.A cikin wannan binciken, an kafa guda ɗaya a gaban endotoxins.Ana kwatanta wannan samuwar gudan jini da sarrafawa don ƙayyade ƙaddamarwar endotoxin.
Yin amfani da bututun gilashi marasa endotoxin yana taimakawa don tabbatar da cewa ganowar endotoxin daidai ne.Wannan saboda endotoxins na iya mannewa saman bututun gilashi kuma suna tsoma baki tare da sakamakon binciken.
Don tabbatar da cewa bututun gilashin da aka yi amfani da su a cikin gel clot endotoxin test assay ba su da endotoxin, ya kamata a wanke su da kayan wanka sannan a wanke su da kyau tare da ruwan da ba shi da endotoxin.Bugu da ƙari, ya kamata a haifuwa ta amfani da autoclaving ko bushewar zafi.
A ƙarshe, yin amfani da bututun gilashin-kyauta na endotoxin a cikin gwajin gwajin endotoxin na gel clot yana da mahimmanci don tabbatar da gano ainihin endotoxins.Ya kamata a tsaftace waɗannan bututun da kyau kuma a ba su haifuwa don kawar da duk wani gurɓataccen abu.
Lokacin aikawa: Juni-02-2023
