Recombinant Cascade Reagents Chromogenic Assay

Kit ɗin Gwajin Bioendo rCR Endotoxin (Recombinant Cascade Reagents Chromogenic Assay) yana amfani da fasahar recombinant gene don bayyana Factor C hanya serine zymogen proteases na doki kaguwa amebocyte a cikin eukaryotic Kwayoyin.Reagents na cascade sun haɗa da Factor C wanda za'a iya kunna shi ta hanyar endotoxins na kwayan cuta, Factor B da enzyme proclotting.Endotoxins suna ɗaure su kuma suna canza Factor C zuwa sigar aikin sa, wanda daga baya yana kunna Factor B, wanda kuma yana kunna enzyme proclotting.Enzyme mai kunnawa da ke aiki sannan ya raba chromogenic substrate, yana sakin pNA mai launin rawaya.Ana iya auna pNA da aka saki ta hanyar hoto a 405 nm.Dangane da abin da, za a iya ƙididdige ƙaddamarwar endotoxin na samfurin gwaji.Kit ɗin gwajin endotoxin na rCR baya amfani da kayan tushen dabba, yana kare kaguwar doki.