Gwajin Endotoxin ta Lyophilized Amebocyte Lysate (LAL Reagent)

Gwajin Endotoxin ta Lyophilized Amebocyte Lysate (LAL Reagent)

Abubuwan da aka bayar na LAL ReagentsLyophilized amebocyte lysate (LAL) wani tsantsa mai ruwa ne na sel jini (amebocytes) daga kaguwar doki na Atlantic.
TAL Reagents: TAL reagent wani tsantsa mai ruwa ne na sel jini daga Tachypleus tridentatus.
A halin yanzu, babban samar da LAL/TAL reagents suna cikin Amurka da China.

Gel clot endotoxin gwajin gwajin, wannan hanyar har yanzu ita ce babbar aikace-aikace a duk faɗin duniya, don biyan buƙatun aminci na magungunan allurar ɗan adam.
A halin yanzu Bioendo yana samarwa kuma yana samar da gel clot LAL reagent ciki har da ampules na gilashin gwaji guda ɗaya da filayen gwaji da yawa.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 da G52
Wannan shine mafita na tattalin arziki don gano endotoxins masu inganci a cikin gwajin magunguna.Musamman ga magungunan allura ko magungunan mahaifa don gwada endotoxins a cikin WFI, API ko samfuran magunguna da aka gama.Gwajin gwajin Endotoxin babban buƙatun ayyukan aiki ne, yana buƙatar ƙwararren mai aiki don tuntuɓar gwajin jini na gel don tabbatar da sake dawowa daidai.

Cikakken bayani gaG52Binciken Endotoxin:
LAL Reagent
Sarrafa Standard Endotoxin
BET Ruwa
Tukwici na pipette-free endotoxin
Bututun gilashi marasa kyauta na Endotoxin, gami da aikin dilution da bututun dauki.
Kayan aikin haɓakawa, don bada shawarar wankan ruwa ko busassun zafi incubator.Duk na'urorin shiryawa suna buƙatar madaidaicin zafin jiki.
Duk abubuwan da ake amfani da su don taɓa gwajin LAL wanda ya dace da ma'aunin matakin kyauta na endotoxin ”<0.005EU/ml” .
Yanayin gwaji zai dace da gano endotoxin.

Kula da:
Abubuwan da ake amfani da su na filastik kamar tukwici na pipette ko faranti na multiwell ana amfani da su sosai don gwajin endotoxin.Pharmacopoeias masu jituwa (USP/CP) suna buƙatar duk wani abin da ake amfani da su na filastik da bututun gilashin dole ne su kasance ba tare da endotoxin da za a iya ganowa ba kuma daidai da mahimmanci amma galibi ba a la'akari da su ba, dole ne su kasance daidaitattun abubuwan da ba su da alaƙa.

Yadda za a yi aiki da gwajin gwajin endotoxin don gano endotoxins a cikin samfurin?
Da farko, ya kamata a yi gwajin don tabbatar da alamar lysate hankali.Don tabbatar da hankali iri ɗaya ne ga alamun alamar.
Don nazarin samfurin, gudanar da gwajin gwajin endotoxin kafin shiga tsakani.
Don sarrafa cikakken gwajin endotoxin na "gwajin tsangwama".
Don gudanar da gwajin iyaka don samun yadda matakin endotoxins yake a cikin samfurin.

Lokacin aiki gwajin don tabbatar da labeled lysate hankali, sakamakon ba al'ada, 2 Lamada havenot gel samuwar?
Bincika ko hanyar shirye-shirye na daidaitaccen sarrafa ƙwayar cuta endotoxin daidai ne.
Ana buƙatar haɗawar Vortex don kowane mai narkewa (duba jagorar koyarwa ko kuma ake kira saka ma'aunin endotoxin daki-daki).
Don bincika ko lysate reagent ya dace da daidaitaccen endotoxin na sarrafawa.

Ana ba da shawarar yin amfani da wanka na ruwa ko busassun zafi mai zafi don tsarin zafin jiki na yau da kullun na reagent lysate (alal misali, incubator ko busassun akwati ba su da tasiri).
A lokacin shiryawa, yanayin ruwa a cikin wanka na ruwa wanda yake a tsaye kuma yana da ƙarfi, ana guje wa duk girgizar ƙasa.
Bayar da kulawa ta musamman ga wankan ruwa don kashe fam ɗin ruwa mai gudana.

Kuna hukunta sakamakon, a hankali cire bututun gwaji ko gilashin ampoule daga ma'aunin zafi da sanyio, a hankali juya digiri 180,
Samfurin gel a cikin bututu ba zai lalace ba, kuma ba zai zamewa ba, an rubuta sakamakon ta sigina "+";
ba a samu samuwar gel ba ko kuma ba za a iya kiyaye ɗigon gel ɗin ba ko da yake an kafa shi.
Zamewar bangon ba ta da kyau, an rubuta sakamakon ta sigina "-".
Kit ɗin gwajin gel clot endotoxin na gaggawa yana cikin hanyar gwajin gel clot endotoxin.
A cikin hanzarin gel clot endotoxin gwajin, samfurin ingantaccen iko ba ya haifar da gel?
Na farko, yi amfani da ruwan gwajin kwayoyin endotoxin na kwayan cuta don tabbatar da lysate reagents, tabbatar da masu aiki da muhalli sun cika ka'idoji;

Idan kit ɗin ya cancanta, to, ingantaccen iko na samfurin ba zai haifar da gel ba saboda tasirin hanawa na samfurin, kuma samfurin ya kamata a sarrafa shi kuma a bi da shi.
Hanyar sarrafa samfurin da aka fi sani shine dilution.