KCET- Kinetic Chromogenic Endotoxin Test Assay (Kwayoyin gwajin endotoxin na Chromogenic hanya ce mai mahimmanci don samfurori tare da wasu tsangwama.)
Gwajin endotoxin na motsi na chromogenic (KCT ko KCET) wata hanya ce da ake amfani da ita don gano kasancewar endotoxins a cikin samfurin.
Endotoxins abubuwa ne masu guba da ake samu a bangon tantanin halitta na wasu nau'ikan ƙwayoyin cuta, gami da ƙwayoyin cuta gram-korau kamar Escherichia coli da Salmonella.A cikin kimar KCET, an ƙara ƙaramin chromogenic zuwa samfurin, wanda ke amsawa tare da kowane endotoxins da ke akwai don samar da canjin launi.
Ana kula da ƙimar haɓakar launi na tsawon lokaci ta amfani da spectrophotometer, kuma ana ƙididdige adadin endotoxin a cikin samfurin bisa ga wannan ƙimar.
Gwajin KCT sanannen hanya ce don gano endotoxins a cikin magunguna, na'urorin likitanci, da sauran samfuran da ke haɗuwa da jikin ɗan adam.Gwaji ne mai mahimmanci kuma abin dogaro wanda zai iya gano ko da ƙananan adadin endotoxin, yana mai da shi kayan aiki mai mahimmanci don tabbatar da amincin waɗannan samfuran.
TAL/LAL reagent shine lyophilized amebocyte lysate wanda aka fitar daga shuɗin jinin Limulus polyphemus ko Tachypleus tridentatus.
Endotoxins sune amphiphilic lipopolysaccharides (LPS) da ke cikin jikin kwayar halitta na waje na kwayoyin gram-korau.Kayayyakin iyaye da aka gurbata da pyrogens ciki har da LPS na iya haifar da haɓakar zazzabi, ƙaddamar da amsa mai kumburi, girgiza, gazawar gabbai da mutuwa a cikin ɗan adam.
Don haka, ƙasashe a duniya sun tsara ƙa'idodi, waɗanda ke buƙatar duk wani samfurin magani da ke da'awar bakararre da marasa pyrogenic yakamata a gwada kafin a sake shi.Gel-clot TAL assay an fara haɓaka shi don gwajin endotoxins na kwayan cuta (watau BET).
Koyaya, wasu ƙarin hanyoyin haɓaka na TAL assay sun fito.Kuma waɗannan hanyoyin ba kawai za su gano ba amma kuma za su ƙididdige kasancewar endotoxins a cikin samfurin.Baya ga fasahar gel-clot, dabarun BET kuma sun ƙunshi dabarar turbidimetric da dabarar chromogenic.Bioendo, wanda aka sadaukar don gano endotoxin, shine ƙwararrun masana'anta don haɓaka ƙirar TAL/LAL na chromogenic.
Bioendo EC Endotoxin Test Kit (Karshen-maki Chromogenic Assay) yana ba da ma'auni mai sauri don ƙididdigar endotoxin.
Hakanan muna samar da Kit ɗin Gwajin Bioendo KC Endotoxin (Kinetic Chromogenic Assay) da mai karanta microplate ELx808IU-SN, wanda zai iya tabbatar da dogaro da ingancin gwaje-gwajenku.
Menene fasali naKinetic chromogenic endotoxin test assaydon gwada endotoxins a cikin samfurori?
Gwajin gwajin chromogenic na endotoxin wata hanya ce da ake amfani da ita don gwada endotoxins a cikin samfuran.Yana da fasali da yawa:
1. Ma'auni na Kinetic: Daidai da ma'auni na turbidimetric, kinetic chromogenic assay kuma ya ƙunshi ma'aunin motsi.Ya dogara da amsawa tsakanin endotoxins da chromogenic substrate don samar da samfur mai launi.Ana kula da canjin launi mai tsanani a tsawon lokaci, yana ba da izinin ƙididdige adadin endotoxin a cikin samfurin.
2. Babban hankali: Ƙwararren chromogenic kinetic yana da matukar damuwa kuma yana iya gano ƙananan matakan endotoxins a cikin samfurori.Yana iya auna ma'aunin endotoxin daidai, ko da a ƙananan matakan, yana tabbatar da gano abin dogara da ƙididdigewa.
3. Fayil mai fa'ida: Gwajin yana da fa'ida mai fa'ida, yana ba da damar auna ma'auni na endotoxin a cikin babban bakan.Wannan yana nufin zai iya gwada samfurori tare da matakan daban-daban na endotoxins, wanda ya dace da ƙananan ƙananan ƙididdiga da ƙididdiga ba tare da buƙatar samfurin dilution ko maida hankali ba.
4. Sakamako mai sauri: Ƙwararren chromogenic kinetic yana ba da sakamako mai sauri idan aka kwatanta da hanyoyin gargajiya.Yawanci yana da ɗan gajeren lokacin tantancewa, yana ba da damar gwaji da sauri da nazarin samfuran.Ana iya lura da ci gaban launi a cikin ainihin lokaci, kuma ana iya samun sakamakon sau da yawa a cikin 'yan mintoci kaɗan zuwa sa'o'i biyu, dangane da ƙayyadaddun kayan aiki da kayan aiki da aka yi amfani da su.
5. Automation da daidaitawa: Ana iya yin gwajin ta amfani da tsarin sarrafa kansa, kamar masu karanta microplate ko
endotoxin-takamaiman analyzers.Wannan yana ba da izinin gwaji mai girma da kuma tabbatar da daidaito da daidaitattun ma'auni, rage kuskuren ɗan adam da haɓaka haɓaka.
6. Karfinsu da yawa tare da nau'ikan samfurori daban-daban: Assimenic Chromogenic assay ya dace da nau'ikan nau'ikan samfurori, ciki har da magunguna, kayan aikin halitta, da samfurori na ruwa.Hanya ce mai amfani da za a iya amfani da ita ga masana'antu da aikace-aikace daban-daban inda ake buƙatar gwajin endotoxin.
Gabaɗaya, kinetic chromogenic endotoxin test assay yana ba da hanya mai hankali, sauri, kuma abin dogaro don ganowa da ƙididdigewa.
endotoxins a cikin samfurori.Ana amfani dashi ko'ina a cikin magunguna, fasahar kere-kere, da masana'antar kiwon lafiya don kula da inganci da aminci.
dalilai na kima.
Lokacin aikawa: Yuli-29-2019
