Ana yin gwajin endotoxin na kwayan cuta (BET) a yawancin dakunan gwaje-gwaje na zamani a ƙarƙashin yanayin sarrafawa a matsayin muhimmin abu don guje wa tsangwama.
Dacefasahar asepticyana da mahimmanci lokacin shiryawa da diluting ka'idoji da samfuran kulawa.Gowningyin aiki a waje da na yau da kullun na kayan aikin kariya na sirri (PPE) Bukatu ba su da damuwa sai dai idan samfurin da ke ƙarƙashin gwaji ya buƙaci takamaiman la'akari da aminci na manazarta saboda guba ko kamuwa da cuta.safar hannuya kamata ya zama marasa TALC, saboda TALC na iya ƙunsar mahimman matakan endotoxins.Masu karatun farantin karfe, ruwan wanka, da busassun busassun zafiYa kamata a yi amfani da shi don samfurin shiryawa ya kamata ya kasance a kan benci na dakin gwaje-gwaje nesa da dumama, iska, da iskar kwandishan (HVAC), rawar jiki mai mahimmanci, da zirga-zirgar dakin gwaje-gwaje wanda zai iya shafar sakamakon gwajin.Samfurin riƙe lokuta da yanayiya kamata a ƙayyade kuma daga baya a rubuta, idan ya cancanta, don tabbatar da cewa za a iya samar da ingantaccen sakamakon gwaji a cikin lokacin da ya dace.
Misali, idan dakin gwaje-gwaje ya sami samfurin Ruwa don allura (WFI) ko samfurin aiki, dole ne a sanya shi a cikin firiji ko zai iya kasancewa a cikin zafin jiki, kuma har tsawon wane lokaci?Kafin gwaji, ana ba da shawarar cewa an haɗa kwantena na farko da kyau kafin cire aliquot(s) gwajin don gwajin kai tsaye ko na gaba.
Bioendo Bacterial Endotoxin Test, gwaje-gwajen sun haɗa dahanyar gel clotgwajin gwajin endotoxinƘididdigar gwajin gwajin endotoxin, Gel clot Hanyar endotoxin gwajin gwaji shine ingantaccen ganowar endotoxin, waɗannan gwaji na buƙatar abubuwan da ake amfani da su shine sarrafa depyrogenation, irin su bututun amsawa kyauta na endotoxin, tubes dilution da tukwici kyauta na pyrogen;Ƙididdigar ƙididdigar endotoxin suna da gwajin chromogenic endotoxin na motsa jiki, gwajin turbidimetric endotoxin, waɗannan gwajin na buƙatar abubuwan da ake amfani da su dole ne su hadu da babban matakin endotoxins ƙasa da ƙasa.0.005EU/ml(0.001EU/ml), irin su tubes na kyauta na endotoxin, tukwici na kyauta na pyrogen, da pyrogen free microplates, har ma da tafki kyauta.Af, idan samfuran jiyya, akwati dole ne ya zama kwalban samfurin kyauta na endotoxin.
A cikin gwajin endotoxin, tsangwama na iya tasowa daga tushe iri-iri, kamar samfuran matrix abubuwan da aka gyara, reagents na gwaji, ko kayan aiki.
Don guje wa tsoma baki na gwaji, ana iya ɗaukar matakai masu zuwa:
1. Shirye-shiryen Samfurin: Shirye-shiryen samfurin da ya dace yana da mahimmanci don gwada gwajin endotoxin daidai.
Ya kamata a gwada matrix samfurin sosai kuma a inganta shi don tabbatar da dacewa tare da gwajin endotoxin.
Musamman ma, abubuwan da ke shiga tsakani kamar lipids da furotin ya kamata a cire ko rage su ta amfani da dabarun da suka dace kamar tacewa ko centrifugation.
2. Gudanarwa mai kyau da mara kyau: Yana da mahimmanci a haɗa da sarrafawa mai kyau da mara kyau a cikin binciken don saka idanu don tsangwama.
Ingantattun sarrafawa suna tabbatar da aikin gwajin, yayin da mummunan sarrafawa ke gano duk wata cuta ko tsangwama daga abubuwan tantancewar.
3. Quality Control: Quality iko ya kamata a yi a kan duk reagents, kayan aiki, da ruwa amfani a cikin assay.
Wannan yana tabbatar da cewa reagents sun sami 'yanci daga gurɓataccen endotoxin kuma suna aiki daidai.
4. Daidaitawa: Ya kamata a daidaita ma'auni don tabbatar da cewa duk sakamakon ya kasance daidai da sake sakewa.
Wannan ya haɗa da yin amfani da madaidaicin lanƙwasa don daidaita ƙima da kuma amfani da daidaitattun dabaru don shirye-shiryen samfurin, ƙaddamarwa, da ganowa.
5. Tabbatarwa: Yakamata a tabbatar da tantancewar don tabbatar da cewa ta keɓantacce, mai hankali, kuma abin dogaro.
Wannan ya haɗa da gwada samfurori da dama, ciki har da waɗanda aka sani da suna dauke da endotoxin, don ƙayyade daidaito da daidaitattun ƙididdiga.
Ta bin waɗannan matakan, za a iya rage tsangwama, kuma za a iya samun ingantaccen gwajin endotoxin.
Lokacin aikawa: Dec-01-2022
