Ruwan da ba shi da endotoxin yana taka muhimmiyar rawa a cikin daidaito da amincin aikin gwajin gwajin endotoxin.Endotoxins, wanda kuma aka sani da lipopolysaccharides (LPS), abubuwa ne masu guba da ke cikin bangon tantanin halitta na kwayoyin Gram-korau.Waɗannan gurɓatattun na iya haifar da mummunar cutarwa ga mutane da dabbobi idan ba a cire su daga kayan aikin likita kamar alluran rigakafi, magunguna, da na'urorin likitanci ba.
Don ganowa da ƙididdige matakan endotoxin daidai, gwajin endotoxin ya dogara ne akan ƙima mai mahimmanci wanda ke buƙatar amfani da ruwan da ba shi da endotoxin.Ana kula da irin wannan nau'in ruwa don cire duk alamun endotoxins, tabbatar da cewa duk wani sakamako mai kyau da aka samu ta hanyar binciken ya kasance ne kawai saboda kasancewar endotoxins a cikin samfurin da ake gwadawa, kuma ba sakamakon gurɓataccen ruwa ba.
Yin amfani da ruwan da ba shi da endotoxin kuma yana taimakawa wajen rage sakamako mai kyau na ƙarya, wanda zai iya faruwa lokacin da akwai adadin endotoxins a cikin ruwan da aka yi amfani da shi a cikin binciken.Wannan na iya haifar da sakamakon da ba daidai ba, mai yuwuwar haifar da jinkiri a cikin sakin samfur da al'amurran da suka shafi tsari.
A taƙaice, ruwan da ba shi da endotoxin wani muhimmin abu ne na aikin gwajin gwajin endotoxin, yana tabbatar da daidaito da amincin wannan gwaji mai mahimmanci.Ta hanyar rage haɗarin ƙididdiga na ƙarya da kuma tabbatar da cewa an samar da sakamako mai kyau kawai a gaban ainihin gurɓataccen endotoxin, ruwan da ba shi da endotoxin yana taka muhimmiyar rawa wajen tabbatar da cewa samfurori na kiwon lafiya suna da lafiya da tasiri don amfani da marasa lafiya.
Bacterial endotoxin gwajin ruwa
Bambanci tsakanin kwayar cutar endotoxin na kwayan cuta da ruwa mara kyau don allura: pH, endotoxin na kwayan cuta da abubuwan tsangwama.
Bacterial endotoxin gwajin ruwa
Bambanci tsakanin kwayar cutar endotoxin na kwayan cuta da ruwa mara kyau don allura: pH, endotoxin na kwayan cuta da abubuwan tsangwama.
1. pH
Mafi dacewa pH don amsawa tsakaninLAL reagentkuma endotoxin shine 6.5-8.0.Sabili da haka, a cikin gwajin LAL, Amurka, Pharmacopoeia na Japan da bugu na 2015 na Pharmacopoeia na kasar Sin sun nuna cewa ƙimar pH na samfurin gwajin dole ne a daidaita shi zuwa 6.0-8.0.Ana sarrafa ƙimar pH na ruwa don gwajin endotoxin na kwayan cuta gabaɗaya a 5.0-7.0;Ya kamata a sarrafa ƙimar pH na ruwa mara kyau don allura a 5.0-7.0.Tunda yawancin magunguna suna da rauni acidic, ƙimar pH na ruwa don gwajin endotoxin na kwayan cuta yana da kyau ga gwajin gwajin endotoxin ko gwajin gwajin Lyophilized amebocyte lysate.
2. Bacterial Endotoxin
Adadin endotoxin a cikin ruwa don gwajin endotoxin na kwayan cuta ya kamata ya zama aƙalla ƙasa da 0.015EU a kowace 1ml, kuma adadin endotoxin a cikin ruwa don gwajin endotoxin na kwayan cuta a cikin ƙididdiga ya kamata ya zama ƙasa da 0.005EU a kowace 1ml;Ruwan da bakararre don allura yakamata ya ƙunshi ƙasa da 0.25 EU na endotoxin a kowace 1ml.
Endotoxin a cikin ruwa don gwajin endotoxin na kwayan cuta dole ne ya zama ƙasa kaɗan wanda bai kamata ya shafi sakamakon gwajin ba.Idan an yi amfani da ruwan da bakararre don allura maimakon gwajin ruwa don gwajin Endotoxin, saboda babban abun ciki na endotoxin a cikin ruwa mara kyau don allura, ruwan da bakararre don allura da Superposition na endotoxin a cikin samfurin da aka gwada na iya haifar da tabbataccen ƙarya, haifar da asarar tattalin arziƙi kai tsaye. zuwa kamfani.Saboda bambancin abun ciki na endotoxin, ba zai yiwu a yi amfani da ruwa mai tsabta don allura ba maimakon ruwan dubawa don gwajin gwajin endotoxin ko Lyophilized amebocyte lysate test assay.
3. Abubuwan Tsangwama
Ruwan don gwajin endotoxin na kwayan cuta dole ne ya tsoma baki tare da reagent na LAL, sarrafa daidaitaccen endotoxin da gwajin LAL;babu bukatar ruwa mai tsafta don allura.Ruwan da bakararre don allura yana buƙatar aminci da kwanciyar hankali, amma ruwan da bakararre don allura zai yi tasiri ga aiki da kwanciyar hankali na daidaitaccen sarrafa ƙwayoyin cuta na endotoxin?Shin Ruwan Bakararre don allura yana haɓaka ko Hana gwajin endotoxin?Mutane kalilan ne suka yi dogon bincike akan wannan.An tabbatar da shi ta hanyar bincike cewa wasu ruwa mara kyau don allura yana da tasirin hanawa mai ƙarfi akan gwajin LAL.Idan an yi amfani da ruwa maras kyau don allura maimakon gwajin ruwa don gwajin LAL, ƙila za a iya haifar da mummunan sakamako, wanda zai haifar da gano endotoxin da aka rasa, wanda ke yin barazana ga amincin magani kai tsaye.Saboda kasancewar abubuwan tsangwama na ruwa mai tsafta don allura, ba zai yiwu a yi amfani da ruwa mai tsafta don allura ba maimakon ruwan dubawa don gwajin LAL.
Idan ana iya tabbatar da daidaiton ruwan wanka, hanyar wankewa da ruwan gwaji, yiwuwar cewa ba za a iya tabbatar da ingantaccen iko a cikin gwajin Limulus ba a zahiri, sai dai in ba a daidaita ma'aunin da aka yi amfani da shi ba.Domin tabbatar da daidaiton sakamakon gwajin, dole ne mu:
a.Wanda ya saba da ka'idoji da ka'idojin masana'antu;
b.Yi amfani da ƙwararrun samfuran da daidaitattun samfuran;
c.Yi aiki daidai da hanyoyin aiki.
Lokacin aikawa: Yuli-26-2023
