Aikace-aikacen Dabarar Chromogenic zuwa Gwajin Endotoxins na Bacterial

Dabarun Chromogenic na daga cikin dabaru guda uku wadanda kuma ke dauke da fasahar gel-clot da dabarar turbidimetric don gano ko tantance endotoxins daga kwayoyin cutar Gram-negative ta amfani da amoebocyte lysate da aka fitar daga shudin jinin kaguwar doki (Limulus polyphemus ko Tachypleus tridentatus).Za a iya rarraba shi azaman ƙimar ƙarshen-chromogenic ko kimantawar kinetic-chromogenic dangane da ƙa'idar tantancewar da aka yi amfani da ita.

Ka'idar amsawa ita ce: lysate amebocyte yana ƙunshe da cascade na serine protease enzymes (proenzymes) wanda endotoxins na kwayan cuta za su iya kunnawa.Endotoxins yana kunna proenzymes don samar da enzymes da aka kunna (waɗanda ake kira coagulase), na ƙarshen yana haifar da rarrabuwa na tsaka-tsakin mara launi, yana sakin samfurin pNA mai launin rawaya.Ana iya auna pNA da aka saki ta hanyar hoto a 405nm.Kuma shayarwa tana da alaƙa da alaƙa da haɗin gwiwar endotoxin, to ana iya ƙididdige ƙwayar endotoxin daidai da haka.